Suppressive effect of pregnant serum on murine dendritic cell function.
Bozorgmehr M, et al.
J Obstet Gynaecol Res. 2012 Mar 22. doi: 10.1111/j.1447-0756.2011.01803.x. [Epub ahead of print]
Affiliation
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University Nanobiotechnology Research Center, Avicenna Research Institute, ACECR Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
Aim: Tolerance to the semi-allogenic fetal graft by the maternal immune system is a medical enigma. Many aspects of immunoregulation at the feto-maternal interface have been clarified, but systemic effects of pregnancy on the immune system are still elusive. The present study was undertaken to determine whether mid-pregnancy mouse serum has an inhibitory effect on dendritic cells (DC) function. Material and Methods: Mid-gestational sera were obtained from allogenic pregnant Balb/c mice (Balb/c × C57BL/6) on days 9-11 of gestation. Splenic DC were purified from Balb/c mice, and treated with mid-pregnancy mouse serum. Antigen pulsed DC were injected into mice palms. After 5 days, draining lymph nodes were removed, cultured in the presence of cognate antigen, and proliferation of responding cells was measured by (3) H-thymidin incorporation. Interleukin (IL)-10 and interferon-gamma (IFN-γ) production by stimulated lymph node antigen-specific cells was also measured in culture supernatants using sandwich ELISA. Results: Treatment of DC with pregnant mouse serum markedly blocked their ability to induce antigen-specific lymphocyte proliferation and IFN-γ and IL-10 production by primed lymph node cells in comparison with non-pregnant serum-treated DC. Conclusion: Pregnant mouse serum has an inhibitory effect on DC capacity to induce antigen-specific proliferation and cytokine secretion by lymph node cells. The suppressive effects of pregnant serum on DC could be considered as one of the mechanisms responsible for the systemic immunomodulation observed during pregnancy.
supported by
CHILDREN GRoW UP CLINIC Yudhasmara Foundation Inspirasi Orangtua Cerdas, Tumbuhkan Anak Semakin Sehat, Kuat dan Pintar
CHILDREN GRoW UP CLINIC I Jl Taman Bendungan Asahan 5 Bendungan Hilir Jakarta Pusat 10210, phone (021) 5703646 – 44466102- CHILDREN GRoW UP CLINIC II MENTENG SQUARE Jl Matraman 30 Jakarta Pusat 10430, phone (021) 44466103 – 97730777
- email :
- http://childrengrowup.wordpress.com
WORKING TOGETHER FOR STRONGER, SMARTER AND HEALTHIER CHILDREN BY EDUCATION, CLINICAL INTERVENTION, RESEARCH AND NETWORKING INFORMATION . Advancing of the future pediatric and future parenting to optimalized physical, mental and social health and well being for fetal, newborn, infant, children, adolescents and young adult
LAYANAN KLINIK KHUSUS “CHILDREN GRoW UP CLINIC”
PROFESIONAL MEDIS “CHILDREN GRoW UP CLINIC”
|
Clinical – Editor in Chief :
Dr WIDODO JUDARWANTO SpA, pediatrician
- email :
- curriculum vitae
- For Daily Newsletter join with this Twitter https://twitter.com/WidoJudarwanto
Information on this web site is provided for informational purposes only and is not a substitute for professional medical advice. You should not use the information on this web site for diagnosing or treating a medical or health condition. You should carefully read all product packaging. If you have or suspect you have a medical problem, promptly contact your professional healthcare provider
Copyright © 2012, CHILDREN GRoW UP CLINIC Information Education Network. All rights reserved
